Principles of chromosomal organization: lessons from yeast

The spatial organization of genes and chromosomes plays an important role in the regulation of several DNA processes. However, the principles and forces underlying this nonrandom organization are mostly unknown. Despite its small dimension, and thanks to new imaging and biochemical techniques, studies of the budding yeast nucleus have led to significant insights into chromosome arrangement and dynamics. The dynamic organization of the yeast genome during interphase argues for both the physical properties of the chromatin fiber and specific molecular interactions as drivers of nuclear order

Physicalization from Theory to Practice: Exploring Physicalization Design across Domains

Currently, physicalization research is dominated by technology-centric explorations with limited insights into the broader domain implications. The goal of this workshop is to bring together researchers and practitioners who share an interest in using data physicalizations to solve real-world problems. Hence, we aim to further explore the utility of physicalization for different domains that (already) apply data physicalization in their practices (e.g., sustainability, office vitality, education, and personal informatics). The objective of the workshop is to combine the expertise of researchers working in physicalization and/or exemplar domains to (i) develop an understanding of common challenges, (ii) map out overarching factors across domains, (ii) operationalize design strategies for common domains, and (iv) reflect on the implementation of data physicalizations for different domains. Upon completion of our workshop, we plan to create a BIT Special Issue addressing the challenges and potential directions of the domain application of data physicalizations

16. Workshop Be-greifbare Interaktion

Tangible interaction is centered around the manipulation of physical objects and the usage of the own body. It thus involves the environment and the physical context much stronger than purely visual or speech-based interfaces. The wide range of possibilities for integrating sensors and computing systems into the physical environment provides ample design space. The research field of Tangible Interaction investigates this scope scientifically and practically in order to enable meaningful and human-oriented applications. In this workshop, the German Informatics Society (GI) specialist group "Be-greifbare Interaktion" of the Department of Human-Computer Interaction offers a forum for the presentation of scientific discourse and interdisciplinary discussion. Contributions range from theoretical, critical and forward-looking reflections to design work and reports on practical implementations. The workshop opens the discussion to a broader audience of experts in order to disclose current developments and generate new impulses for the research field

Systematic characterization of the conformation and dynamics of budding yeast chromosome XII

International audienceChromosomes architecture is viewed as a key component of gene regulation, but principles of chromosomal folding remain elusive. Here we used high-throughput live cell microscopy to characterize the conformation and dynamics of the longest chromosome of Saccharomyces cerevisiae (XII). Chromosome XII carries the ribosomal DNA (rDNA) that defines the nucleolus, a major hallmark of nuclear organization. We determined intranuclear positions of 15 loci distributed every ~100 kb along the chromosome, and investigated their motion over broad time scales (0.2-400 s). Loci positions and motions, except for the rDNA, were consistent with a computational model of chromosomes based on tethered polymers and with the Rouse model from polymer physics, respectively. Furthermore, rapamycin-dependent transcriptional reprogramming of the genome only marginally affected the chromosome XII internal large-scale organization. Our comprehensive investigation of chromosome XII is thus in agreement with recent studies and models in which long-range architecture is largely determined by the physical principles of tethered polymers and volume exclusion